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Bulgarian Journal of Physics
Print: 1310-0157 Online: 1314-2666
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RECOGNIZED BY THE EUROPEAN PHYSICAL SOCIETY 
Bulg. J. Phys. vol.36 no.2 (2009), pp. 139-144
Atomic Absorption Assessment of Mineral Iron Quantity in Ferritin
M. Marinova1, L. Vladimirova2
1National Diagnostic Veterinary Research Institute ``Prof. Dr. Georgi Pavlov'', 15, Pencho Slaveykov Blvd., 1606 Sofia, Bulgaria
2University of Sofia ``St. Kliment Ohridski'', Faculty of Physics, Department of Atomic Physics, 5 James Boucher Blvd. 1164 Sofia, Bulgaria
go back1National Diagnostic Veterinary Research Institute ``Prof. Dr. Georgi Pavlov'', 15, Pencho Slaveykov Blvd., 1606 Sofia, Bulgaria
2University of Sofia ``St. Kliment Ohridski'', Faculty of Physics, Department of Atomic Physics, 5 James Boucher Blvd. 1164 Sofia, Bulgaria
Abstract. Possibilities for quantitative determination of the number of iron atoms in the mineral core of ferritin by atomic absorption spectroscopy (AAS) are investigated in the work. Different measurements with AAS show an iron content from 1000 up to 4500 atoms per molecule ferritin [1-6]. This motivated us to investigate the amount of iron in the Horse Spleen Ferritin with atomic absorption spectroscopy under application of the Bulgarian standard BDS EN 14082/2003 Foodstuffs - Determination of trace elements - Determination of lead, cadmium, zinc, copper, iron and chromium by atomic absorption spectrometry (AAS) after dry ashing. The obtained results give aprox. 1800 atoms per molecule Ferritin. It is in accordance with previous results, published by leading researchers [4,5]. The investigation of the iron content with AAS under the use of the Bulgarian standard is a good opportunity to study many other objects of biological interest.